Study of transgenic SlRAPx rice plants

• Main Authors: Yu-Chun Liu, 劉育羣
• Other Authors: Dr. Ting-Feng Wu
• Format: Others
• Language: zh-TW
• Published: 101
• Online Access: http://ndltd.ncl.edu.tw/handle/75633367969451230262

碩士 === 南台科技大學 === 生物科技系 === 100 === A tomato RAP2.6-like transcription factor, SlRAPx, belong to AP2/ERF family. Previous studies have found that members of this AP2 family involved in the regulation of plant disease resistance and salt tolerance in plant. To determine the function of SlRAPx we transfer this gene into rice by Agrobacterium–mediated transformation. Several transgenic plants, seven T1 trnasgenic rice and seven T2 trnasgenic rice plants, had been obtained by hygromycin selection from 19 T0 trangenic rice. The foreign gene has been integrated into rice genome proven by Southern blot analyses. The expression level of SlRAPx gene was also highly expressed in transgenic rice plants. However, carry out the (methyl viologen，MV) test, these SlRAPx transgenic plants was not significant tolerance compared to wild type. In the near future, we will determine the tolerant ability of various stresses in these transgenic rice plants.