| Summary: | 碩士 === 南台科技大學 === 生物科技系 === 100 === In out previous studies, the difference of serum estrogen levels between the female and male rats resulted in the better heat tolerance in the former than the latter. However, there have been no studies in heat stress tolerance in pregnancy. In the literatures, experimental heat stroke (HS) induced whole body inflammation and vascular endothelial damage. As for prenatal inflammation and ischemia, they primarily lead to be considerably crucial factors for neonatal cerebral in jury and cerebral palsy. We hypothesize the lethality in pregnant mice after exposing to heat stress were the most crucial. Therefore, we set up the heatstroke pregnant animal model in this study.
Unanesthetized, unrestrained mice (the male, proestrus, and pregnant ICR mice) were exposed to ambient temperature, 40℃; relative humidity, 50℃-55℃; 55 min, in an environment-controlled chamber to induce HS. Survival rate after heat stress among the male, proestrus, and pregnant mice will be compared. The gestational day (gd)-14 pregnant mice were defined as the pregnant mice.
We compare male mice, non-pregnant and pregnant mice as their tolerate to heat stoke and analyzed the post heat stroke survival rate. We found that after endure 4 hours of heat stroke, pregnant mice have the highest lethality compared to male and non-pregnant mice. This further demonstrated that pregnant mice have a low tolerance for exposure to extended period of heated temperature. At the same time, we analyze markers for cellular and neuronal damage plus scoring for organ dysfunction (under HE stain, TUNEL stain), pregnant mice after exposed in a heat stress will sustain multi-organ failure (eg. Hypothalamus, spleen, liver, kidney and lung), the degree of injury is far more severe than male and non-pregnant mice. In conclusions, our data indicated tat heat tolerance would be worst in the pregnant mice compared with male and non-pregnant mice. In addition, there is presence of severe ischemic encephalopathy and multi-organ dysfunction in the pregnant mice after exposure to heat stress.
|
|---|
