Studies On The Antioxidant Activity Constituents Of Bischofia Javanica Flower (II)

• Main Authors: Hsu, Weichen, 許瑋真
• Other Authors: Huang, Kehfeng
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/22224025790859960941

碩士 === 靜宜大學 === 應用化學系 === 100 === Bischofia javanica Blume., Euphorbiaceae, is a cultivated species in Taiwan as a roadside tree, and had the anti-ulcer, the expelling worms and anti-dysenteric activites. The antioxidant activity of 80% ethanol extract and its subfractions of Bischofia javanica flower, was evaluated using the DPPH free radical scavenging ability.The total phenolic content of theses extracts was determined as gallic acid equivalent. The total flavonoids content of theses extracts was determined as quercetin equivalent. The ethyl acetate soluble fraction and n-butanol soluble fraction of Bischofia javanica flower possessed the higher DPPH free radical scavenging, (EC50=70.40 μg/mL and 70.46 μg/mL, respectively). The total phenolic content were estimated as 965.30 mg/g gallic acid and 640.03 mg/g gallic acid, respectively. The total flavonoids content were estimated as 514.87 mg/g flavonoids and 318.30 mg/g flavonoids, respectively. The active components were obtained from column chromatography, namedly, gallic acid, isocorilagin, quercetin-3-O-rutinoside, and naringenin. The structures were elucidated by spectroscopic methods and comparison with an authentic sample.