Phytochemical study on Rhoeo discolor

• Main Authors: Chun-Yu Chen, 陳群諭
• Other Authors: Shyh-Yuan Li
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/57401411524971858707

碩士 === 中國文化大學 === 化學系應用化學碩士班 === 100 === Rhoeo discolor (L'Her.) Hance (Commelinaceae) is a well-know folk medicine in taiwan. The whole herbs of this plant are used to treat in flammation, bruising, pneumonia, cough and liver fibrosis, etc. In this study, the chemical constituents of the whole plant of R.discolor were investigated and their anti-inflammatory and anti-cancer activities were tested. Air-dried studied material was extracted with 95% EtOH at 55℃ for 24 hr. After evaporation of the solvent in vaco, the extract was trented with CH2Cl2-Me2CO (1:1) and then MeOH to obtain two soluble portions. They were individually and purified by a combination of several chromatographic techniques, including led to the isolation of nine compounds which were (R)-3-(3,4-dihydroxyphenyl)-5-hydroxy-5H-furan-2-one (1), (R)-3-(3,4-dihydroxyphenyl)-5-methoxy-5H-furan-2-one (2), 3-(3',4'-dihydroxyphenyl)-5H-furan-2-one (3), 4-((E)-3-(3,4-dihydroxyphenyl)acryloyloxy)-2,3-dihydroxy-2-methylbutanoic acid (4), (E)-ferulic acid hexacosyl ester(5), quercetin-3-O-β-D-glucoside(6''→1''') -α-L-arabinoside (6), L-adenosine (7), 3,4-dihydroxybenzoic acid (8) and compound 9. The structures of isolated compounds were established by application of spectroscopic analyses (NMR, MS, UV, IR). Among these compounds, 1, 2 and 3 are new compounds, as well as 4 and 6 are first derived from natural source. The preliminary results of showed that the CH2Cl2-Me2CO(1:1) soluble portion extracts of the stem, flower and leaf of R.discolor displayed dose-dependent anti-inflammatory effect at 1, 10, 50 and 100μg/mL on lipopolysaccaride(LPS)-induced nitric oxide (NO) production in RAW 267.7 macrophages. Their EC50 values were 51.81, 46.85, 85.48 μg/mL, respectively. The TC50 values of MTT assay were greater than 1000 μg / mL. The above three extracts and compounds 1, 2, and 6 showed no significant anti-cancer activities on MCF-7 (breast), NCl-H460 (lung), HT-29(colon) and CEM (leukemia) cancer cells.