Physicochemical Property of the Carboxyl Methyl Cellulose/Collagen Hydrogels Containing Siver Nanoparticles

• Main Authors: Lai,Ying-Ru, 賴瀅如
• Other Authors: Yao,wei-Hua
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/96924394712229803210

碩士 === 亞東技術學院 === 應用科技研究所 === 100 === The study prepared silver nanoparticles by NaBH4 chemical reduction method, and it were added to blending hydrogel by carboxymethyl cellulose/collagen (CxCoy) to prepare CxCoyAgz solutions and membranes at last by adjusting pH and concentration of silver nanoparticles, and analyzed with UV-Vis, FTIR-ATR spectrum, DSC, mechanical properties, SEM morphology and antibacterial test. UV-Vis spectrums show that 0.5wt%C3Co1 possesses minimum value (81.23) of full width at half maximum and supreme absorbance (1.91) in alkali. The ionic polymer will hold ample COO- in alkali, and it could adsorb and chelate with Ag+, and then reduce abundant silver nanoparticles disperse in polymer well. FTIR-ATR spectrums reveal that C1Co1 membrane owns best interaction, cause of approximate hydrogen bond sites and highly bond strength (376 cm-1). Due to addition of Ag+, it could occupy partial hydrogen bond sites cause membrane bond strength to decrease, while (C1Co1)1.0Ag1 at pH 5 and (C1Co1)0.5Ag1 at pH 11 membrane provide with the highest bond strength 372 cm-1 and 371 cm-1. DSC thermal characterizations investigate that C1Co1 membrane with highest Tg, Tm and enthalpy, and addition of 0.3mM of Ag into CxCoy membrane is higher. High interaction makes silver nanoparticles couldn’t blend with hydrogel well, deposit on surface only and cause high Tm (133℃) and enthalpy. Mechanical property tests indicate that C1Co1 membrane has the highest tensile strength (42.87MPa) and elongation (30.4%). Young’s modulus and tensile strength increase with concentration of silver nanoparticles decreases in alkali. (C1Co1)1.0Ag1 membrane has high young’s modulus (57.8) and tensile strength (51.6MPa)among the results, but its elongation (7.2%) is the lowest. Many silver nanoparticles deposited on surface of membrane and high interaction made high young’s modulus and tensile strength, but it became brittleness and low elongation at the same time. SEM morphology shows that once add nanoscale silver to membrane which will make minute particles deposit on its surface. Antibacterial test results reveal that added silver nanoparticles to CxCoy membrane and contacted with Staphylococcus aureus, inhibition rates are 95-100% for 3 hours and 100% for 24 hours. As a result, CxCoy blending ratio 1:1 as the best silver particles stabilizer.