Application of Graphene Oxide in Purple Membrane Chip Technology

• Main Authors: Aline Natasia Kosasih, 許遠玲
• Other Authors: Hsiu-Mei Chen
• Format: Others
• Language: en_US
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/mn7ywe

碩士 === 國立臺灣科技大學 === 化學工程系 === 100 === The objective of this research was to explore the integration of graphene oxide (GO) with purple membrane (PM) in the approach for a novel bio-sensing system. As the oxidized form of graphene, GO possesses unique and stable structure derived from graphene. In addition, it is relatively cheap and easy to produce. PM from Halobacterium salinarum contains only one protein constituent, bacteriorhodopsin (BR) that acts as a light-driven proton pump providing novel photonic properties. A facile way to introduce GO into PM chip technology was developed with evaluation of the GO addition effect by AFM, contact angle, and photocurrent analyses. GO-avidin complex was prepared and subsequently used as a linker to immobilize biotinylated PM onto ITO fabricated with an amine-terminated self-assembled monolayer. Morphological studies conducted with AFM revealed that GO addition through GO-avidin complex enhanced surface flatness of the PM chips and the amount of GO employed was critical to yield a distinguishable effect on the surface. Nevertheless, no significant inhibition of photoelectric responses generated from PM was observed on GO addition. The bioaffinity immobilization through biotin-avidin interaction was found to be important for high PM coverage, because the nonspecific binding directly between GO and PM yielded less stable and lower photoelectric responses. In addition, GO alone was integrated into the PM chip fabrication during the layer-by-layer assembly. GO was added with different coating methods prior to avidin and subsequently b-PM immobilization. The contact angle analysis confirmed the presence of coated GO in each method, while the chip photocurrents were reduced by GO integration. In the future, GO-integrated PM biochips might lead to a bioassay with unique and enhanced properties.