A poloxamer / chitosan in situ forming gel for bone tissue engineering

• Main Authors: Thai-Kim-Thi Vu, 武蔡金詩
• Other Authors: Ming-Chien Yang
• Format: Others
• Language: en_US
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/24629237949761612920

碩士 === 國立臺灣科技大學 === 材料科學與工程系 === 100 === This work is to develop an in situ gelation scaffold for bone tissue engineering to restore and maintain the function of human bone tissues. To meet this goal, the gel is comprised of F127 (a poloxamer) and chitosan. The gelation temperature (Tgel), viscoelastic properties and mucoadhesive force of the systems were investigated and optimized by means of rheological analyses. At specific concentrations, the resulting thermoreversible gel exhibited a Tgel close to the body temperature. The addition of chitosan did not hamper the self assembling process of F127, only elevating the gelation temperature. Furthermore, chitosan can improve the rheological properties of the gel, possibly due to the interaction between chitosan and F127 micelles through hydrogen bond, which reinforced the gel structure. The DSC results showed the interactions between water and F127 gels, indicating the influence of chitosan on the physicochemical features of gels. TGA results also indicated the interactions between the gels and water. Mucoadhesion experiments showed a rheological synergism between F127/chitosan gels and mucin dispersion, changing the flow behavior from a Newtonian fluid to a pseudoplastic fluid. In vitro release experiments indicated that the optimized gel was able to prolong and control acyclovir release for more than 20 min. Biocompatibility of the gel of F127 and chitosan was observed based on cell proliferation, protein adsorption and complete blood count test.