| Summary: | 碩士 === 國立臺灣大學 === 臨床牙醫學研究所 === 100 === Aim：Both Stem cells from human exfoliated deciduous teeth (SHED) and Stem cells from apical papilla (SCAP) are multipotent stem cells. After neural induction, these two kinds of cells could be differentiated into neuron like cells. We hypothesized that SHED and SCAP have the same neurodifferentiation potentials. The purpose of this study is to compare the differences of neurodifferentiation potentials of SHED and SCAP.
Materials and Methods：We cultivated SHED and SCAP in neural induction medium for 0 day, 1 day ,3 days, 7 days , 14 days and 21 days and analyzed cell morphology, cell proliferation, gene expression patterns(RT-PCR) and immunofluorescence before and after differentiation.
Results：After 3 days cultivation in neural induction medium, the morphology of SHED and SCAP were changed to neuron like cells. We found that cell proliferation of SHED and SCAP were reduced. After 7 days cultivation in neural induction medium, gene expression patterns (RT-PCR) and Immunofluorescence analysis of the expression Neurofilament demonstrated that both SHED and SCAP were successfully differentiate into neuron cells. However, the Neurofilament gene expression of inducted SCAP was much upregulated then that of inducted SHED. This result indicated that the neurodifferentiation potential of SCAP is higher than that of SHED.
Conclusion ：Although both SHED and SCAP have potentials for neurodifferentiation but neurodifferentiation potential of SCAP is much better than that of SHED. We can expect that by using SCAP for neurogenesis would get better results.
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