Study on the Anti-inflammatory Effect of Mesenchymal Stem Cells in Murine Model of Collagen-Induced Arthritis

• Main Authors: Ning-Ling Huang, 黃甯翎
• Other Authors: 江伯倫
• Format: Others
• Language: en_US
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/27508184058553892087

碩士 === 國立臺灣大學 === 口腔生物科學研究所 === 100 === Background: Rheumatoid arthritis (RA) is a common autoimmune disease in the world. A variety of immune cells are involved in pathogenic mechanism and progression of the disease. In the early stage of RA, T lymphocytes are regarded as one of crucial cells in the disease pathogenesis and are related to disease severity. Mesenchymal stem cells (MSCs) are pluripotent stem cells obtained from many tissues. MSCs can self-renew and differentiate into several lineages under stimulations. Recently, more and more studies have demonstrated that MSCs exert the immunoregulatory capacity. In this study, we aimed to examine whether MSCs can have potential therapeutic effect on collagen-induced arthritis (CIA) in rheumatoid arthritis murine model. Methods: We isolated MSCs from bone marrow of DBA/1J mice. MSCs were expanded, characterized by phenotypic analysis, and differentiated into osteocytes, chondrocytes, and adipocytes. Proliferation of T lymphocytes in the absence or presence of MSCs was performed to determine the immunosuppressive function of MSCs in vitro. To investigate the effect of MSCs on CIA animal model, we immunized mice with bovine type II collagen, and injected MSCs intravenously. Results: MSCs efficiently inhibited the anti-CD3/28-induced T cell proliferation and division. These data suggested that MSCs had the regulatory effect on inflammatory immune responses. MSCs administration can reduce the severity of arthritis and cell infiltration. The level of IL -1β was lower in the sera of MSC-treated mice than non MSC-treated group. IL-6 expression in knee protein extraction was decreased after mice receiving MSCs. Furthermore, the lymphocytes of MSC-treated mice secreted higher IFN-γ, IL-6, and IL-10. Conclusions: Our results demonstrated that MSCs could play a modulatory role in the immune responses both in vitro and in vivo. The delivery of MSCs in CIA animal model ameliorated the severity of arthritis. MSCs treatment can be used as a potential therapy for RA. However, further studies are required to clarify the immunoregulatory mechanisms mediated by MSCs treatment.