The immunomodulatory effect of polyphenol-enriched areca nut extract in a murine model of food allergy

• Main Authors: Yu-Ju Lin, 林玉茹
• Other Authors: 詹東榮
• Format: Others
• Language: en_US
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/76qcww

碩士 === 國立臺灣大學 === 獸醫學研究所 === 100 === Arecae semen, the slice of dried areca nut, has been used in traditional Chinese medicine for the treatment of intestinal parasitosis, diarrhea and tenesmus. However, limited scientific evidence is available to substantiate its pharmacological effects. Previous studies from this laboratory showed that areca nut extract (ANE) influenced delayed-type hypersensitivity reactions, suggesting that the T-cell mediated immunity was affected by areca ingredients. In addition, ANE induced the generation of myeloid-derived suppressor cells, characterized as CD11b+Gr-1+ cells. This thesis study investigated the immunomodulatory effect of polyphenol-enriched ANE (PANE) in a murine model of food allergy. For intraperitoneal (IP) administration, BALB/c mice were daily administered with PANE (5-25 mg/kg in sterile saline; 0.1 mL/mouse) from day 1-3, day 15-17 and day 29-31. For oral administration, mice were administrated with 0.05% and 0.1% (w/v; approximate 5 mL/mouse/day) of PANE in drinking water throughout the entire experiment. Except for the naive and non-sensitized group, the mice were systemically sensitized with ovalbumin (OVA) on day 3 and day 17. Except for the NA group, mice were repeatedly challenged with OVA (50 mg/0.3 mL in saline/mouse) by gavage from day 31 to 49 to induce allergic responses. Administration of PANE did not affect the body weight, spleen index, and the cellularity of splenocytes. Both IP injection and oral uptake of PANE attenuated food allergic responses, including diarrhea incidence, infiltration and degranulation of mast cells and the inflammatory pathological change in the intestine. PANE significantly reduced the number of IL-4+ cells in the duodenum, but dose-dependently increased the infiltration of IL-10-producing Gr-1+ cells. Furthermore, the number of IFN-γ+ cells in the duodenum and the serum production of total IgE and OVA-IgG1 were attenuated by IP treatment with PANE. Collectively, these results demonstrated the anti-allergic effect of PANE, which is associated with the suppression of T cell-mediated immunity and the induction of MDSC generation.