Luminol as light source in (meso-tetraphenylporphyrin)-mediated photodynamic therapy

• Main Authors: Ling Huang, 黃翎
• Other Authors: Feng-Hui Lin
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/18180621541796935007

碩士 === 國立臺灣大學 === 醫學工程學研究所 === 100 === Due to the limitation of the penetration depth of light, photodynamic therapy is mainly applied to treat cancers that occur on the surface of the body, such as skin, neck, and oral cavity cancers. If we want to apply photodynamic therapy to treat cancers in the inner body, such as colon cancer, we would have to deliver the light in some special ways, for example, by using optical fibers along with endoscopes to deliver the light to the inner body. However, colonoscopy might be uncomfortable for most patients mentally and physically. Also, light cannot penetrate into the tumor with large volume, which reduces the effectiveness of treatment. In order to expand the application of photodynamic therapy in the inner body, we used luminol as the light source to activate the porphyrin family photosensitizer, meso-tetraphenylporphyrin (TPP), to achieve photodynamic therapy. From experiment, we could know that luminol and FeSO4 did not cause damages to cells on day 1, and cells incubated with DMSO or TPP showed slightly decrease in cell viability. On day 3, we found that the effects of luminol, DMSO, and TPP on cells were more obvious than day 1 and there were also slight increases in cytotoxicity in comparison with the control group. Among those experimental groups, PDT treated group had the lowest cell viability and highest cytotoxicity on both day 1 and day 3, which showed that cells were seriously damaged and further died in large amounts. We had the similar results from the flow cytometry and confocal microscope outcome. In the analysis of cell death pathway, we found that cell had shrunk after PDT treatment, which is a feature of apoptosis. However, we could not observe early apoptosis signals from the Annexin V-FITC/PI staining results. We thought that the incorrect timing to capture early apoptosis signals may be the cause of this phenomenon. Therefore, we could not make a clear conclusion from the data. In brief, we thought that luminol is a promising light source in the inner body, and we would continue to adjust the experimental parameters for better treatment results.