Genes expression and immune responses in orange spotted grouper (Epinephelus coioides) following vaccination against grouper iridovirus (GIV) infection

• Main Authors: Bin-Hong Hsu, 徐賓鴻
• Other Authors: Chi-Yao Chang
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/76318925873917627228

碩士 === 國立臺灣大學 === 漁業科學研究所 === 100 === Grouper iridovirus (GIV) can infect all life stages in grouper and cause serious death. It also make low survival rate in grouper from fry to juvenile stage, and may cause aquaculture economic damage. To develop a high efficacy vaccine is important to resist iridovirus . Calculating the relative percent survival (RPS) rate post challenge and detecting the titer of specific antibody and its maintain efficiency, are used to estimate the efficacy of vaccine in the past. But the interaction between host and iridovirus is not clear completely. According microarray results in GIV infection orange-spotted grouper (Epinephelus coioides) , we selected many candidate gene in our lab previously. Thus, we have confirmed and analyzed 30 virus responsive genes (VRGs) in response to innate immunity and adaptive immunity in grouper’s head kidney and spleen after GIV infection. In this study, we analyzed the expression of 15 VRGs in grouper following vaccination against GIV infection by intraperitoneal injection. We discovered that DHX58, HECT, Mx, Viperin, ISG15, PLAUR, CD9 and VLIG1 expression patterns are very similar, and these genes are considered to involving in interferon regulated pathway in innate immunity. On the other hand, most VRGs expression are lower in vaccination group than in control after GIV challenge. The maximum anti-GIV specific antibody is produced in fish sera post vaccination one week, even in the group of low dose vaccine treatment. The ELISA results showed that host immune response by vaccination exist dose-dependent relationship. These results indicated that the neutralization between ant-GIV specific antibody and GIV caused viral load reducing in the vaccinated group after GIV challenging.