The Micropropagation of Nothapodytes nimmoniana

• Main Authors: An-Po Hun, 洪恩保
• Other Authors: 王亞男
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/88220399884390054595

碩士 === 國立臺灣大學 === 森林環境暨資源學研究所 === 100 === The Immature Nothapodyes nimmoniana seeds were harvested in July. The seeds were sterilized sonicated for 10 min with 1% sodium hypochlorite aqueous solution after washing, the sterilization rate was 84%. The zygotic embryos were sown on MS medium in aseptic environment for cultivating aseptic seedlings. The apical buds and stem segment of Nothapodyes nimmoniana aseptic seedling were used as explants for multiple buds inducing test. The optimal formula for multiple buds inducting test was MS medium and 0.3mg/L PGR TDZ. The induting rate on apical buds and stem segment were 90% and 83% respectively. Multiple buds were cut into 1cm segment for shoot elongation. The optimal formula for shoot elongation was MS medium, 0.2mg/L BA and 0.1mg/L IBA. Elongated shoots were used as explants for roots induction. The optimal formula for root induction was 1/4MS, 0.5mg/L IBA and 1mg/L IAA. Shoots with root were used in adaptation. The survival rate was 55%.