Impacts of Recombinant Paenibacillus Cellulase Pretreatment on Pulp Refining Characteristics

• Main Authors: Chien-Ying Yang, 楊千瑩
• Other Authors: 柯淳涵
• Format: Others
• Language: en_US
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/35473714027373520255

碩士 === 國立臺灣大學 === 森林環境暨資源學研究所 === 100 === This study aimed at the progress of application of cellulase in refining, including mechanism of action on cellulose and their effects on physical properties on paper. Utilization of enzymes becomes a popular biotechnology, and there are more and more researches confirm their potential. Refining is a necessary process to form the paper where the cellulosic fibers are mechanically treated, resulting into morphological and structural changes to produce desired fiber properties for a better quality paper. However, refining is energy-cost stage in pulp and paper industry. For this reason, the effect of cellulase was evaluated for energy saving in refining process of papermaking. The recombinant Paenibacillus cellulase was used to improve the efficiency of the refining process of two pulps, using different enzyme dosages and different PFI revolutions. The pulps used in this work were the commercial bleached Eucalyptus globulus kraft pulp and Pseudotsuga menziesii kraft pulp. This treatment increased drainability of both pulps at the same level of refining energy. In other words, that consumed less refining revolutions to reach the same drainability compared to the untreated pulps. Although the viscosity of both pulps were degrade with the enzymatic treatment, the physical properties of paper had no significant negatively influence on them. Moreover, the samples were observed by Scanning electron microscopy (SEM), and analyzed the roughness and height of fiber by Atomic force microscopy (AFM).　The results showed the treatment with cellulase swelled the fibers in the absence of refining, and there were better fibrillation on the fibers treated with cellulase after refining. Furthermore, the statistical analysis of AFM suggested the both pulps treated with low cellulase dosage with PFI refining had higher roughness.