Analyze and identify the anti-inflammation and anti-tumor compounds from Chinese olive extracts through cell-based platform mediated functional evaluation

• Main Authors: YI-An Chen, 陳奕安
• Other Authors: 謝淑貞
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/06510517705345223131

碩士 === 國立臺灣大學 === 食品科技研究所 === 100 === Epidemiological studies have shown a lower incidence of atherosclerosis, cardiovascular diseases and certain kinds of cancer in the Mediterranean countries Olive oil is a characteristic component of the Mediterranean diet and offers beneficial effects, such as antioxidative activity, antimicrobial, reducing lipoprotein, reducing platelet agglutination, anti-inflammation, and activity for regulating cell proliferation. Chinese olive (Canarium album L.) belongs to Burseraceae family which differs from Mediterranean diet’s olive in type. It is also a traditional medicine material, exhibiting beneficial functions in anti-bacterium, anti-virus, anti-inflammation and detoxification. Previous studies have displayed Chinese olive a phenolic compounds rich resource for providing antioxidative activity. However, the role of Chinese olive in anti-cancer and anti-inflammation remains unknown. Therefore, our aim is to search for compounds with anti-proliferation or anti-inflammation effect in Chinese olive .In our study, we used WST-1 assay and anti-inflammation platform as tools to examine the biological function of compounds within Chinese olive, and in parallel, the functional extract of Chinese olive has beenr enriched and then analyzed by HPLC-MS-MS. We conclude that the olive methanol-EA fraction extract has the best anti-inflammation and anti- proliferation effects when compared with the other feactons. Using solid phase extraction to separate methanol-EA fraction into 11 sub-fractions, we found that sub. fr. 2, sub. fr. 7 have the strongest anti-inflammation and anti- proliferation effects. Methyl gallate is the main compound in sub. fr. 2, while unknown compound with molecular weight 538 is the main compound in sub. fr. 7. Therefore, we hypothesize that Methyl gallate and unknown compound with molecular weight 538 might be the functional compounds offering both anti- inflammation and anti- proliferation effects in Chinese olive exteact.