Effect of phenolic acids on ameliorating damage of mouse brain neuroblastoma after experiencing ischemia-like oxygen glucose deprivation

• Main Authors: Hong-Dian Cheng, 鄭弘典
• Other Authors: Swi-Bea Wu
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/49076113322304369322

碩士 === 國立臺灣大學 === 食品科技研究所 === 100 === Cerebral ischemia, also known as Stroke, is a disease caused by the rapidly loss of blood supply to brain and which might affect intellectual function such as memory, language skills, perception, or cognitive skills including reasoning and judgment. Many studies have shown that a variety of phenols reduce brain damage on rats induced by brain ischemia and reperfusion. Therefore, the purpose of our study is to find some potential phenols to improve neuron damage in vitro. Oxygen glucose deprivation (OGD), a cell model approximating the conditions associated with cerebral ischemia in vivo, induces cell damage by reducing the supply of oxygen and glucose. The brain requires a continuous supply of oxygen and glucose to maintain normal function and viability. In our study, 10 μM ρ-coumaric acid could increase 14.97% of cell viability and 1 μM Chlorogenic acid increase 9.49% of cell viability after experiencing ischemia-like OGD damage. These two phenolic acids, 10 μM ρ-coumaric acid and 1 μM Chlorogenic acid, decrease 40.71% and 25.80% percentage of autophagy. 10 μM ρ-coumaric acid treatment also elevated 24.90% of intracellular calcium concentration and 1 μM Chlorogenic acid raised 88.65% of intracellular calcium concentration compared with OGD damage alone. We supposed that increase in cell viability of these two phenolic acids was through ameliorating autophagy, and the pathway they regular autophagy was elevating intracellular calcium concentration.