| Summary: | 碩士 === 國立臺灣大學 === 物理研究所 === 100 === After years of development, laser has been applied in numerous medical treatment procedures. Surgeries such as correcting near and far sightedness, dissection on soft tissue and even cancer-relate therapies are gradually being implemented. Choosing different power, repetition, wavelength of the laser, we can create multifarious chemical or physical reactions with different bio-materials. In our experiment, we use 780nm, femtosecond laser to dissect neuron cell (PC12) and the touch neuron of Caenorhabditis elegans, in order to observe the following behaviors after excising distinct cellular componentss.
Femtosecond pulse laser embraces plenty of advantages in surgical purposes. The repetition rate of our laser pulse is 80-MHz, and the maximum cutting power is 20mW. Since Ti-sapphire laser provides high energy in each laser pulse, we can cut neurons by tuning pulse energy around 0.25nJ, without extensive tissue damage. In addition, we use near infrared light as source (780nm wavelength), pushing the focus deeper into biological specimens. Therefore, we can successfully execute precise surgery on the micron-sized sample such as collagen, cells or even the specific parts of neuron.
Combining two optical systems, we confirm the position of C.elegans neuron by GFP, and meanwhile, use Ti-Sapphire laser to dissect the targeted neuron section . After witnessing the damage on neuron, we use a picker to motivate the exercise of C.elegans and to study its post-surgery behaviors.
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