Intermolecular binding between TIFA-FHA & TIFA-pT9 mediates TNF-alpha stimulation & NF-kappaB activation

• Main Authors: Chia-Chi Huang, 黃家琦
• Other Authors: Ming-Daw Tsai
• Format: Others
• Language: en_US
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/17013842990059578404

博士 === 國立臺灣大學 === 生化科學研究所 === 100 === The forkhead-associated (FHA) domain recognizes phosphothreonine (pT) with high specificity and functional diversity. TIFA (TRAF-interacting protein with a FHA domain) is the smallest FHA-containing human protein. Its over-expression was previously suggested to provoke NF-kappaB activation, yet its exact roles in this signaling pathway and the underlying molecular mechanism remain unclear. Here we identify a novel phosphorylated threonine site, threonine 9 (pT9), on TIFA and show that this phosphorylation site binds with the FHA domain of TIFA, leading to TIFA oligomerization and TIFA-mediated NF-kappaB activation. Detailed analysis indicated that unphosphorylated TIFA exists as an intrinsic dimer, and that the FHA-pT9 binding occurs between different dimers of TIFA. In addition, silencing of endogenous TIFA resulted in attenuation of TNF-alpha-mediated downstream signaling. We therefore propose that the TIFA FHA-pT9 binding provides a previously unidentified link between TNF-alpha stimulation and NF-kappaB activation. The intermolecular FHA-pT9 binding between dimers also represents a new mechanism for the FHA domain.