| Summary: | 碩士 === 國立臺北教育大學 === 自然科學教育學系碩士班 === 100 === Lichtheimia and the other related genera (Absidia and Lentamyces) also known as the group : Absidia sensu lato. There are ubiquitously distributed saprotrophic fungi, which can be isolated from soil, leaf litters, dung or compost.
In this study, 1385 soil, 77 composts and 333 dung samples were collected from Taiwan. Totally, 53 strains were isolated from soil, 10 strains from composts and 28 strains from dung. Based on morphological characters, 8 species, Absidia cylindrospora, A. glauca, A. repens, A. spinosa, Lichtheimia corymbifera, L. hyalospora, L. ornata and L. ramosa, are classified. Lichtheimia and Absidia cultures were also obtained from BCRC and Germany Jena University laboratory which were used as references. Molecular biotechnology including polymerase chain reaction (PCR), gene cloning and sequencing were used to obtain the sequences of ITS region (ITS1-5.8S-ITS2), LSU-D1/D2 domain and SSU of ribosomal DNA (rDNA). The ultrastructure observed in the homothallic zygospore of A. spinosa, including scanning electron microscope (SEM) and transmission electron microscope (TEM).
This study found that in the Absidia globose spored group, for the same species the rDNA sequences from the Taiwanese strains and European strains are identical, however, are considerable difference in the cylindrical spored group. The situation of Lichtheimia is similar with globose spored Absidia group. The ultrastructural observation of zygospore use light microscope and electron microscope, it had interspace between zygosporangium and zygospore, zygospore with a thick wall thickness of about 2.5-3 μm.
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