Validation of Wnt Signaling Regulators Identified by a Previously Performed shRNA Screen

• Main Authors: Nam Jiun Zhi, 藍浚智
• Other Authors: Chung-Leung Li
• Format: Others
• Language: en_US
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/84752999886489025643

碩士 === 國立臺灣海洋大學 === 生物科技研究所 === 100 === Wnt signaling pathway is one of the essential pathways playing various regulatory roles in embryonic development, cell fate determination and tissue homeostasis throughout the life of the organism. Furthermore, dysregulated Wnt activation has been observed in various diseases including colon cancer and leukemia. The pathway gains more attention as it poses possibilities for discovering new drug targets and medical advances. Despite numerous studies have been done on Wnt signaling, the pathway network is found to be extraordinarily complex and yet to be completely understood. Thus, searching for novel regulator of Wnt signaling pathway is important for expanding our knowledge on the pathway and its partner. Previously, by combining RNAi knockdown and reporter system, we have obtained a number of Wnt regulator candidates. In this study, forced expression of the candidates in Wnt reporter system and 3T3-L1 differentiation assay indicates that these selected candidate genes, Ccrk, Cdk7 and Mtmr6, have no effect on Wnt activity. Lastly, we find that the ΔN115ΔC751 β-catenin shows an impaired Wnt activating capability and may have a role in regulating adipogenic differentiation of 3T3-L1 cells.