Preparation and Characterization of Dual - Mode Imaging Technology and Folate - Mediated Multifunctional Shellfish Wastes Transfer into Hydroxyapatites

• Main Authors: Tzu-Yi Yu, 游子毅
• Other Authors: Hsiu-Mei Lin
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/69338360562527020671

碩士 === 國立臺灣海洋大學 === 生物科技研究所 === 100 === In this study, we used the hydrothermal conversion method to modify the shellfish waste, and make it change into hydroxyapatite (HA). Both the clam shell and freshwater clam shell are the two of major economic shellfish and shell. Shell conversion of hydroxyapatite has a fine biocompatibility and bone re-sorption. We assemble the materials that doped with rare earth lanthanide element, europium (Eu3+) and gadolinium (Gd3+), and the multifunctional Eu3+/ Gd3+ dual-doped hydroxyapatite (HA) has both fluorescent and magnetic properties of bioimaging and magnetic resonance (MR) imaging. We used surface modification to graft folic acid on the surface of the material that doped with europium (Eu3+) and gadolinium (Gd3+) with targeting characteristics. Because of folic acid receptors are over-expressed in human cancer cell, so it can be used to target the cancer cell. In vitro experiments on cellular uptake of the folate-conjugated multifunctional HA by using HeLa cancer cells and L929 fibroblast cells to promote cell phagocytosis. HeLa cells are used to the targeting effect because of the over-expression of folate receptors on the cell surface, and L929 cells with low folate receptor are used as a negative control. By confocal microscopy to observe the material grafted folic acid for the cancer cells targeting. It is confirmed to the shellfish waste transfer into our materials can have a multi-functional potential of dual-mode imaging technology and folic acid targeting.