| Summary: | 碩士 === 國立臺灣海洋大學 === 食品科學系 === 100 === 15 g (dry weight) S. siliquosum sequentially treated with 121℃ hot water extraction for 40 min, 0.4 N HCl hydrolysis, 13,680 U cellulase hydrolysis, 100 U MA103 and MAEF108-Sar crude enzymes hydrolysis, then inoculated 12% yeast strains BCRC 21686 + 21687 + 22220 fermentated at 18℃ for 3 day, initially obtain 18.15 g/100 g of ethanol yield. Yeast salt tolerance test have shown that yeast strains BCRC 21824 + 22510 both can tolerate salinity (60 psu), and a combination of this two yeast strains can efficient using the simple sugars in the S. siliquosum hydrolyzate (21.12 mg/mL) to produce 1.06% of ethanol concentration (residual sugar, 4.75 mg/mL). Furthermore, the optimal total inoculation amount and the optimal fermentation temperature of this yeast combination are 12% and 25℃, respectively. 15 g (dry weight) S. siliquosum sequentially treated with 121℃ hot water extraction for 20 min, 0.4 N HCl hydrolysis, 13,680 U cellulase hydrolysis, 22,000 U amylase hydrolysis, 100 U MA103 and MAEF108-Sar crude enzymes hydrolysis, then inoculated 12% yeast strains BCRC 21824 + 22510 fermentated at 25℃ for 3 day, further obtain 21.90 g/100 g of ethanol yield. In addition, 15 g (dry weight) S. siliquosum sequentially treated with 121℃ hot HCl (0.4 N) extraction for 20 min, 13,680 U cellulase hydrolysis, 22,000 U amylase hydrolysis, 100 U MA103 and MAEF108-Sar crude enzymes hydrolysis, then inoculated 12% yeast strains BCRC 21824 + 22510 fermentated at 25℃ for 3 day, finally obtain 25.48 g/100 g of ethanol yield. The fed-batch fermentation test have shown that ethanol fermentation of S. siliquosum hydrolyzate by yeast strains BCRC 21824 + 22510 at 25℃ for 5 day, can make the ethanol yield of 25.48 g/100 g increased to 26.67 g/100 g, this have tremendous potential for future development. The high performance liquid chromatography have shown that S. siliquosum sequentially treated with hot HCl extraction, cellulase hydrolysis, amylase hydrolysis, MA103 and MAEF108-Sar crude enzymes hydrolysis can let the extracted S. siliquosum oligosaccharides hydrolysis into simple sugars, which could be used to fermentated to produce ethanol by yeast strains BCRC 21824 + 22510.
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