Prophylactic effects of the chitosan-encapsulated DNA vaccine in combination with multiple CpG by oral vaccination against Helicobacter pylori infection

• Main Authors: Fang-Ting Kuo, 郭芳廷
• Other Authors: Chang-Jer Wu
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/12125217135411306923

碩士 === 國立臺灣海洋大學 === 食品科學系 === 100 === Helicobacter pylori is recognized as the main cause of chronic gastritis, ulcers and gastric cancer in humans. With increased incidence of treatment failure and antibiotic resistance, development of prophylactic or therapeutic vaccination is a desirable alternative. This study focused on the gastric and systemic immune responses induced by DNA vaccination combination with the multi-CpG adjuvant in the H. pylori infection mice. Vaccine-induced protection was correlated with the increased proportions of CD4+ T cells, IL-17, neutrophils and the decreased percentage of Treg in the gastric mucosa. The C3H/HeN mice orally immunized by H. pylori DNA vaccine/chitosan complexes can induce specific and protective antibodies against H. pylori challenge. Analysis of serum cytokines and the percentage of splenocytes revealed that DNA vaccination combined with the adjuvant can induced a better Th1-biased immune response than that without adjuvant. The prophylactically oral-immunized mice with chitosan-pCJ-3/UreB or pCJ-3/Hsp60 DNA vaccine reached 31.25% and 37% protective rates, respectivity. When combining with the adjuvant, the protective rate increased to 75%. These results demonstrate that DNA vaccine through oral immunization exerts its prophylaxis against the pathogen.