Ameliorating Effects of Petri Dish Cultured Antrodia cinnamomea Fruiting Body Toward Colon Cancer Cachexia and Inflammatory Bowel Disease in Murine

• Main Authors: Chien-Sheng Wu, 吳建勝
• Other Authors: Zwe-Ling Kong
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/79149067854532162183

碩士 === 國立臺灣海洋大學 === 食品科學系 === 100 === Inflammatory bowel diseases (IBD) are associated with chronic relapsing inflammation of the intestinal tract. IBD are at increased risk of developing CRC since it is now known that 90–95% of the cause is attributable to lifestyle and diet. Many studies have confirmed that Antrodia cinnamomea have antioxidant and anti-inflammatory and protect liver property. But, anti-inflammatory effects of AC in IBD have not been investigated. First,we use petri dish cultured AC fruiting body water extract (ACP) and ethanol extract (ACE) was assessed via inhibitory effect of nitric oxide (NO) and tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and prostaglandin E2 (PGE2) in lipopolysaccharide (LPS) induced RAW 264.7 macrophage cells.The mRNA expression levels of interleukin-6 (IL-6) and inducible NO synthase (iNOS) were reduced by the addition of ACP and ACE in a dose-dependent manner. Second , we used dextran sulfate sodium (DSS) (3% W/V) in drinking water fourteen days to induce male C57BL/6 mice colitis, and oral AC powder to mice for last seven days. DSS produced shrinkage of colon length and increased the relative colon weight/length ratio and inducing inflammatory response. AC attenuated the severity of DSS-induced IBD,reducing pro-inflammatory cytokine. In the colitis-associated colorectal cancer (CRC) model, mice were treated with 3 cycle DSS (2% W/V) for colitis and intraperitoneal injection of azoxymethane (10 mg/kg AOM), AC could be in prevent of CRC in the AOM-DSS model. The result shows that AC mitigated the injurious effects of DSS and inhibited pro-inflammatory cytokine and reduced tumor number. These results suggest that AC could possibly have improve injurious effects of DSS and that it may have therapeutic value in the setting of IBD and CAC.