The application of divalent vibrio vaccine and encapsulation of oral vaccine on cultured grouper (Epinephelus sp.)

• Main Authors: Wai-Sang Ip, 葉偉生
• Other Authors: Ping-Chung Liu
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/04216334929963729113

碩士 === 國立臺灣海洋大學 === 水產養殖學系 === 100 === Abstract The present study was designed to investigate the physical properties and immune effects of oral vaccines using encapsulated Vibrio alginolyticus or Vibrio carchariae as sodium alginate microparticles in vaccinated groupers and also to study the immune response of grouper after vaccination with both oil and aluminum adjuvanted bivalent vaccine of Vibrio alginolyticus and Vibrio carchariae delivered via intraperitoneal injection. In the preparation of microparticles vaccine, sodium alginate as coating material could effectively coat the bacterial without any changes in the chemical structure between sodium alginate and also bacteria, therefore could maintain the integrity of the bacteria. Under simulation of gastric fluid condition (pH 3.75), sodium alginate could effectively protect the antigen from destruction while swelling phenomenon of microparticles was observed under simulation of intestine fluid condition (pH 7.4), and thus favored the sustained release of bacteria. The prepared sodium alginate microparticles using 1.5% sodium alginate and 1.5% calcium chloride under the stirring speed of 500 rpm have a spherical shape, even distribution of particle size and good release effect. After vaccination using alginate coated-bacteria microparticle delivered by oral feeding, the protective effect in the grouper and the serum antibody were effectively enhanced with the highest relative percentage survival (RPS) of 75% and 88.89% after challenged with Vibrio alginolyticus (S4Y) and Vibrio carchariae (EMI), respectively. On the other hand, increased immune effects in grouper against Vibrio alginolyticus and Vibrio carchariae were observed after vaccination via intraperitoneal injection with both oil and aluminium adjuvanted bivalent vaccine. From the results obtained, grouper vaccinated with oil adjuvanted vaccine showed the best protective effect with the relative percentage survival (RPS) up to 100% in grouper against Vibrio alginolyticus while that for aluminum adjuvanted vaccine showed RPS of 88.9% in grouper against Vibrio carchariae, and both protective effects could last continuously up to Weeks 24.