| Summary: | 碩士 === 國立臺灣師範大學 === 生命科學研究所 === 100 === Adiponectin which is one of adipokines has attracted much attention in these years because of its significant effects to improve metabolic syndromes, such as type 2 diabetes, coronary artery disease, and hypertension. Nevertheless, the signal transduction pathway of adiponectin remains largely unknown. Utilizing two approaches, yeast two hybrid and immunoprecipitation, we began to explore the AdipoR1 binding partners which might be involved in adiponectin/AdipoR1 signaling pathway. TMEM43 was found in the immunoprecipitation recovery database of MS-SPEC results. Expression of RFP-TMEM43 and GFP-AdipoR1 in many cell types seems to colocalize under fluorescent microscope. Coimmunoprecipitation experiments were further employed to demonstrate that FLAG-TMEM43 significantly binds to GFP-AdipoR1. Previous studies considered that TMEM43 might be activated by PPAR-γ which involved in regulating cellular lipid metabolism in adiponectin/AdipoR1 signaling pathway. It is plausible that TMEM43 could play an important role in adiponectin/AdipoR1/PPARs signaling pathway. Hopefully, this study could provide new therapeutic target(s) for the drugs development to ameliorate metabolism related diseases.
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