Studies of Gd2O3：Eu-AP Nanoparticles for Trimodal MRI/CT/Fluorescence Molecular Imaging

• Main Author: 溫延展
• Other Authors: 陳家俊
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/94500413295674727734

碩士 === 國立臺灣師範大學 === 化學系 === 100 === Recent nano-biomedicine have devote to research the topics for the development of single nano-materials possessing multifunctional properties. In this thesis, we synthesized the multifunctional europiumdoped gadolinium oxide ( Gd2O3:Eu ) nanoparticles which equipped with fluorescence, magnetic resonance signal and X-ray absorption. Then we applied the specific targeting aptamer encapsulated outside the nanoparticles for molecular imaging. First, Gd2O3:Eu nanoparticles were synthesized via the polyol method and were characterized based on TEM, XRD, UV-PL, SQUID and EDS. Then we identified the surface capping ligand citric acid of Gd2O3:Eu nanoparticles through FT-IR, TGA, and zetasizer. Ultimately,we employed qPCR and confocal to confirm the binding of Gd2O3:Eu-AP nanoparticles. Gd2O3:Eu nanoparticles were applied on the in vitro MRI. Compared to the conventional T1 MRI gadolinium chelated complex such as Gd-DOTA、Gd-DTPA（r1 4.1s-1mM-1）, the relaxivity of Gd2O3:Eu nanoparticles（r1 5.0199s-1mM-1）was much higher. In other words, the effect of MRI enhanced contrast image by Gd2O3:Eu nanoparticles was better. On the in vitro CT, there was a much higher HU value of Gd2O3:Eu nanoparticles at the weight concentration of 350 mg/ml than the common CT iodine contrast agent IOHEXOL. But less than 350 mg/ml, the HU value of Gd2O3:Eu nanoparticles was still lower than IOHEXOL.