| Summary: | 碩士 === 國立屏東科技大學 === 熱帶農業暨國際合作系所 === 100 === In an attempt to control the negative grouper factors proliferation, the used of probiotic Saccharomyces cerevisiae P13 which at the moment is widely applied as a dietary supplement in aquaculture has been taken into consideration due to its successful results in the enhancement of growth performance, innate immune response as well as the improvement of disease resistance of finish and crustaceans. S. cerevisiae β-glucans are likely to be the most important compounds in the stimulation of the humoral and cellular immune response in fish. The objectives of this study were to clarify the effect of S. cerevisiae P13 ability to enhance growth performance, the innate immune system such as phagocyte activity, respiratory burst activity, superoxide dismutase (SOD) activity, serum alternative complement activity (ACH50), lysosyme activity, and resistance to Streptoccocus sp. infection in orange-spotted grouper Epinephelus coioides. A total of four different S. cerevisiae P13 basal diet concentrations 103, 105, 107 cfu kg-1 and control diet 0 were orally administrated for a period of four and five months. The mean body weight of the fish supplemented with S. cerevisiae P13 basal diet concentrations 103, 105, 107 cfu kg-1 showed significant results throughout the entire experiment as compared with control diet 0 cfu kg-1 S. cerevisiae P13 ability to enhancement the innate immune system in orange spotted grouper E. coioides was also demostrated as S. cerevisiae P13 basal diet concentrations 103, 105, 107 cfu kg-1 showed significant results in the innate immune response when compared to control diet 0 cfu kg-1. The cellular innate response specifically the phagocytic activity, the respiratory burst of the head-kidney leucocyte, and the superoxide dismutase (SOD) activity which play an important role in the first line defense against the various pathogens were obviously triggered at a significant level compare to control. Such an increment in the cellular innate response in orange spotted grouper E. coioides after fed S. cerevisiae P13 supplemented diet was evaluated once again as the fish were challenge with Streptoccocus sp. infection, in which significant results were obtained by those fish fed S. cerevisiae P13 basal diet concentrations 103, 105, 107 cfu kg-1 compared to control diet 0 cfu kg-1. Therefore, it is concluded that dietary administration of S. cerevisiae P13 basal diet concentrations 103 cfu kg-1 supplemented for four months promoted at an optimum level the growth performance of orange spotted grouper E. coioides. S. cerevisiae P13 basal diet concentrations 107 cfu kg-1 supplemented for five months promoted at an optimum level the enhancement of the innate immune response and the disease resistance to Streptoccocus sp. infection of orange spotted grouper E. coioides.
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