| Summary: | 碩士 === 國立屏東科技大學 === 獸醫學系所 === 100 === Toxoplasmosis, Dirofilariasis, Lyme disease, Leptospirosis and Q fever are important worldwide zoonotic diseases and cause the great loss of economy. The specific aim of this research is to understand the prevalene and epidemiology of zoonotic diseases in Kaohsiung under different factors like age, sex, breed, season, domestic dogs and stray dogs. Blood specimens were collected and subjected to Polymerase Chain Reaction（PCR）to examine the occurrance of these disease. The PCR was revelaed specificity and sensitivity of detection of pathogen DNA. The positive rate of each diseases was as followed: Toxoplasmosis was 0.14% (2/1440), Dirofilariasis was 7.08% (102/1440), Lyme disease was 0.07% (1/1440), Leptospirosis was 0.07% (1/1440), and Q fever was 4.79% (69/1440). According to the statistical analysis, Dirofilariasis infection related with ages, domestic dogs and stray dogs. The elder dogs with higher infected possibility. The stray dogs stung by vector mosquito more easily, thus with higher infection rate than domestic dogs. The environmental temperature was relatively stable in the south of Taiwan, thus, the infection of Dirofilariasis was irrelevant to seasons. Q fever infection was related with ages, seasons and domestic dogs and stray dogs. The highest infection of Q fever was in spring and winter, it should be possible related with reproduction and the spread of pathogens.
By comparsion of gene sequence between isolated strain with the United States, Germany, Brazil, China strain showed a similarity of 98.7%. Heartworm positive strains of Taiwan with China and Iran strain showed similarity of 95-97% and 89-94% respectively. Lyme disease positive strains compared with the United States, Germany, Sweden strain revelaed a similarity of 97-98%. Leptospira positive strains compared with China, Brazil, United States, Japan strain showed a similarity of 98.5-99.5%. Q fever positive strains compared with Japan, Korea, the United States strain showed a similarity of 97-100%.Taken together these results couldbe the useful information for epidemic disease prevention .
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