Detection of Zoonotic Diseases in Domestic Dogs and Stray Dogs by Molecular Diagnostic Techniques in Central Taiwan

• Main Authors: Chun-hung Chou, 周俊宏
• Other Authors: Ming-Hui Liao
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/53160103942271613837

碩士 === 國立屏東科技大學 === 獸醫學系所 === 100 === In recent years, due to the alteration of life styles, many pet owners pay more attention to their dog's health and medicine. Since domestic dogs and stray dogs live in the same environment, zoonoses had been became a serious public health problem. There are five common zoonoses in Taiwan, including toxoplasmosis,heartworm disease,lyme disease,leptospirosis and Q fever.In this study, random sampling of blood samples from domestic and stray dogs in Central Taiwan (including Taichung, Changhua and Yunlin). Blood 720 samples from 30 domestic and 30 stray dogs were collected every month from August 1, 2009 to July 31, 2011. Polymerase chain reaction (PCR)was utilized to diagnosis the five zoonotic diseases and analyze the prevalence. The sensitivity of PCR was downed to 1 pg/μL DNA. The PCR positive rate of those diseases was as followed: in stray dogs group, Toxoplasma gondii 3.89 % (28/720), Dirofilaria immitis 7.22 % (52/720), Borrelia burgdorferi 5.42 % (39/720), Leptospira spp. 7.22 % (52/720), and Coxiella burnetii 5.83 % (42/720), by which in domestic dogs group, Toxoplasma gondii 1.39 % (10/720), Dirofilaria immitis 7.08 % (51/720), Borrelia burgdorferi 3.33 % (24/720), Leptospira spp. 6.25 % (45/720), and Coxiella burnetii 8.61 % (62/720). The total positive rate was Toxoplasma gondii 2.64% (38/1440), Dirofilaria immitis 7.15 % (103/1440), Borrelia burgdorferi 4.38 % (63/1440), Leptospira spp. 6.74 % (97/1440), and Coxiella burnetii 7.22 % (104/1440). In the sequencing and phylogenetic analysis, the isolated Toxoplasma gondii and United States, Germany, Brazil and Chinese strain shared with the similarity of 95–100 %. The isolated strains and Taiwan and China strain contained 96–97 % sequence similarity, Iran contained 93 % sequence identity with Dirofilaria immitis. The isolated strains and United States, Germany and Sweden strain shared 98–100 % sequence identity with Borrelia burgdorferi. The isolated strains and China , Brazil, United States and Japan strain possessed of 97–100 % sequence identity with Leptospira spp. The isolated strains and Japan and United States strain contained 99-100 % sequence identity with Coxiella burnetii. By the statistical data analysis, we concluded: (a)for the stray dogs: Toxoplasma gondii, Borrelia burgdorferi, and Leptospira spp. are correlated with the age and seasons, but not correlated with the breed and gender ; Dirofilaria immitis species are correlated with the breed and seasons, but not correlated with the gender and age ; Coxiella burnetii is correlated with the seasons, but not correlated with the breed, gender and age ; (b)for the domestic dog: Toxoplasma gondii, Borrelia burgdorferi, Coxiella burnetii are correlated with the seasons, but not correlated with the species, gender and age ; Dirofilaria immitis is correlated with the breed, age and seasons, but not correlated with the gender ; Leptospira spp. is correlated with the gender and season, but not correlated with the age and species. The current results revealed the high sensitivity and high specificity of primer pairs by polymerase chain reaction in zoonoses diseases detection, thus, these results could be the useful information as the reference for epidemic disease prevention.