| Summary: | 碩士 === 國立屏東科技大學 === 農園生產系所 === 100 === One problem encountered during commercial production of Phalaenopsis mericlone is the difficulty of in vitro rooting in some cultivars which may lead to low survival rate and decrease the economical value of resultant young plants. The aim of this study was to explore the effect of supplemental chemicals in culture media on adventitious root induction of in vitro grown Phalaenopsis shoots. Four fertilizers with different nitrogen (N), phosphorus (P), and potassium (K) concentrations were used to induce adventitious roots in vitro by use of two months old shoots with 2 leaves. The percent of rooting, mean root number, mean root length and mean leaf number were recorded after two months of culture. Supplementing 3 g/l 10N-30P-20K in the medium increased root formation rate. By fixing the fertilizer dosage, organic supplements, including potato powder, potato or sweet potato were further tested for efficacy of inducing roots. The highest rooting rate was on medium supplemented with potato powder. Higher sucrose concentration increased rooting rate, mean root number and mean root length, with 87 mM the best. All subsequent experiments used optimized sucrose, fertilizer, and potato powder as basal adventitious root induction medium. Adding 5.7μM IAA to the BM increased higher rooting rate. Exogenous calcium chloride and calcium nitrate had no effect on rooting, except promoting shoot growth.
|
|---|
