嗜熱革節孢菌生理特性與其添加於堆肥內對巴西蘑菇菌絲生長及產菇之影響

• Main Authors: Pei-Chun Lien, 連珮君
• Other Authors: Wen-Jinn Liang
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/29901976130767375281

碩士 === 國立屏東科技大學 === 植物醫學系所 === 100 === Scytalidium thermophilum ( =Torula thermophilum ) is an important thermophilic fungus in the composting process of mushroom compost, because it has stimulatory effects on mycelium growth and increases mushroom yield when Agaricus mushroom fungi were cultivated in the S. thermophilum amended composts. The objectives of this research is focused on (1) the physiological and culture characteristics of S. thermophilum, (2) the influence of this thermophilic fungus on pangolagrass composting, and on mycelium growth and mushroom production of Agaricus blazei which was cultivated in S. thermophilum amended pangolagrass compost. The optimal mycelium growth of S. thermophilum was obtained at 45℃, and at pH 7~8. The yeast extract glucose agar (YEGA) and starch agar (SA) were better media for mycelium growth than the other tested media. Sucrose and starch were better carbon source, while asparagine, glutamine and sodium nitrate were better nitrogen source for mycelial growth when tested with Czapek agar basic medium. Oat grain was the best material for spawn preparation among the 5 tested materials. The higher water content of pangolagrass in among the range of 50-80％ after water adding , the better for mycelium growth of S. thermophilum was found. In experiment of the mycelium growth of S. thermophilum in petri dish containing pangolagrass compost harvested at different composting phase, the results showed that this thermophilic fungus could grow on all composts derived from many composting phase, but the best compost was from 6 days after piling. When the pangolagrass composts derived from different composting phase, were first sterilized and then inoculated with S. thermophilum and incubated at 45℃ for 4 days before inoculating A. blazei, the mycelial growth of A. blazei was better on the composts harvested during 7 to 25-day composting than that on composts without inoculation with S. thermophilum; but the unsterilized composts did not show such difference. The mycelium growth of Agaricus blazei in long test tube grown on pangolagrass composts derived from different composting phase were sterilized and inoculated with S. thermophilum and incubated at 45 ℃ for 0、1、3、5 days, the results showed that mycelium growth of A. blazei was better on the sterilized compost with S. thermophilum growth for 1 to 5 days than that of 0 days incubation. The pangolagrass composts harvested at 2nd and 3rd turning of composting process that were inoculated with S. thermophilum and then incubated for 4 days were better for mycelium growth of A. blazei than that on un-inoculated composts. The biological efficiency (%) of compost and mushroom yields of Agaricus blazei cultivated on compost amended with oat spawn or liquid spawn of S. thermophilum were significantly different from that of S. thermophilum un-amended compost. The mushroom yields of Agaricus blazei cultivated on compost spawned with 100g and 200g of oat spawn of Scytalidium thermophilum was not significantly different.