Ursolic acid suppresses TNF-α-mediated activation of NF-κB, IL-6 induced STAT3 activationand and decrease detoxifying enzymes GLO-І leading to apoptosis in HL-60.

• Main Authors: Wan-Ling Chuang, 莊婉伶
• Other Authors: Tzou-Chi Huang
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/17259451649452284112

碩士 === 國立屏東科技大學 === 食品科學系所 === 100 === Ursolic acid (UA) is pentacyclic triterpene acid which is widely using in medical herbs and edible plants. The study focus on effect of UA to induction apoptosis in human promyelocytic leukemia (HL-60) cell lines. This experiment use UA for treatment HL-60 cells by using difference concentration and time. In this study we found Sub-G1 phase cell accumulation is significantly. In the part of DNA fragmentation is observed HL-60 cells treated with 30 μM of UA for 24h.The morphology of apoptotic cell is changes. We founds condensed and fragmented chromatin in UA treated cells .UA treated cell not only can inhibited NF-κB transduction by TNF-α induced but also can decreased Glyoxalase I (GLO- I) expression in various concentration and time. We found that ursolic acid inhibited both constitutive and interleukin-6-inducible STAT3 activation in a dose- and time-dependent manner in HL-60 cells. We successful to using UA to induce apoptosis in human promyelocytic leukemia (HL-60) cell lines. The signal mechanism probably by suppresses TNF-α-mediated activation of NF-κB, consecutively activation of anti-apoptosis protein and IL-6 induced STAT3 activation.