Curcumin induced human colon cancer cell Caco-2 apoptosis through induction of transcription factor PPAR-γ

• Main Authors: Yu-Lin Chang, 張玉麟
• Other Authors: Tzou-Chi Huang
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/33496045470033040377

碩士 === 國立屏東科技大學 === 生物科技研究所 === 100 === Turmeric is a spice common to India and the surrounding regions. The edible part of turmeric is derived from the rhizome of Curcuma longa. Many literatures indicated that curcumin has the anti-inflammatory, anti-oxidant activity, anti-bacteria, anti-viral and enhance wound healing and anti-cancer functions .Pre-clinical studies in a variety of cancer cell lines including breast, cervical, colon, gastric, hepatic, leukemia, oral epithelial, ovarian, pancreatic, and prostate have consistently shown that curcumin possesses anti-cancer activity in vitro and in pre-clinical animal models. In this study, we investigated the mechanism of curcumin-induced apoptosis in Human colon adenocarcinoma cell Caco-2. Treatment of Caco-2 cells with curcumin decreased cell viability and revealed nuclear condensation at concentrations of 50 μM for 48 hrs by both the MTT experiment and Confocal observation. Cell cycle analysis by flow cytometry showed that curcumin induced significant G1 arrest and apoptosis in Caco-2 cells. By using the confocal microscopy PPAR-γ but not NF-κB translocation was observed. We also use the western blot to check the expression of Glyoxalase 1. Curcumin showed dose dependent inhibition effect on Glyoxalase 1 protein expression. The role of PPAR-γ translocation in curcumin induced apoptosis was investigated and discussed.