| Summary: | 碩士 === 國立澎湖科技大學 === 食品科學研究所 === 100 === This study aims to Saccharomycopsis fibuligera PF32 function in rice bran extraction and preliminary study of the enzyme. Base of the purple rice to cooked, using S. fibuligera PF32 and high alcohol production by Saccharomyces cerevisiae NHCUE 03, NHCUE 09, and traditional brewing of peka, comparison between three of the antioxidant activity, reducing sugar and alcohol content, and rice white wine as the control group.
S. fibuligera PF32 the result of fermentation of fresh rice bran, and stability of rice bran and stability of defatted rice bran, the highest total polyphenol content of the three kinds of rice bran were 7.62,12.00 and 11.97 gallic acid eq. mg / g DW, all three about twice higher than the alcohol extraction fold. Highest DPPH scavenging activity of three kinds of rice bran 9.34, 10.33 and 11.98 trolox eq. μmole/g DW, respectively, All three about 5 times higher than the alcohol extraction fold. Highest TEAC antioxidant capacity of three kinds of rice bran25.15, 37.83 and 37.58 trolox eq. μmole/g DW, three than alcohol extraction higher of 3, and 3.5 fold, respectively. We also analyse to S. fibuligera PF32 with laccase, cellulase and β-glucosidase, laccase activity of three highest 344.91, 141.04 and 49.12 U/g protein, respectively, cellulase activity of three highest of 0.62, 0.23 and 0.38 U/ g protein, respectively, β-glucosidase activity of three highest of 0.62, 0.23 and 0.38 U/ g protein. From the above result was informed that S. fibuligera PF32 can effectively improve the antioxidant substances released from rice bran.
Results of purple rice wine, three antioxidant activity than no difference, In total anthocyanidin content of NHCUE 03 and NHCUE 09 lower than the peka. Because pH values influence lead to anthocyanin cracking speed faster. But NHCUE 03 and NHCUE 09 content of alcohol and reducing sugar consumption rate is better than the peka. Three alcohol content were NHCUE 09>NHCUE 03> peak and reducing sugar consumption rate were peak > NHCUE 03 > NHCUE 09. From the above results that, S. fibuligera PF32 and NHCUE 09 brewed rice wine can have output less reducing sugar consumption and higher alcohol content.
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