3-hydroxytetradecanoic acid as B. cenocepacia lipopolysaccharide surrogate related to the degrees of inflammation response using mouse air pouch model

• Main Authors: Chiung-Fang Hsu, 許瓊芳
• Other Authors: Ya-Lei Chen
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/13596479009497629679

碩士 === 高雄師範大學 === 生物科技系 === 100 === Using a murine hypodermic air pouch infection model designed to mimic the release of bacterial products at physiological levels, 3-hydroxy fatty acid (3-OH FA) and endotoxin unit levels from Burkholderia cenocepacia isolates were assessed. The B. cenocepacia environmental isolates (n=35), with independent genetic patterns, survived in the hypodermic air pouch but did not invade across the peritoneal epithelial layer during a 72-h infection. However, cepacia mouse will be estabilished if Balb/c mice were infected with such B. cenocepacia isolates independently by intravascular or peritoneal infection. For all 35 strains, when the molar ratio of C14:0 3-OH FA to C16:0 3-OH FA in the air pouch fluid wash samples was between 1.4 and 2.5, the concentrations of C14:0 3-OH FA were correlated with the endotoxin unit levels (R2=0.6663). However, both surrogate markers exhibited different correlations to the inflammatory response. The linear regression coefficient was 0.4234 for C14:0 3-OH FA concentrations vs. NO productions, 0.223 for endotoxin unit levels vs. NO productions, 0.5008 for C14:0 3-OH FA concentrations vs. TNF-alpha productions and 0.2869 for endotoxin unit levels vs. TNF-alpha productions. Therefore, C14:0 3-OH FA concentrations, rather than endotoxin unit levels, acted as an immunostimulatory indicator for LPS in the B. cenocepacia isolates