| Summary: | 碩士 === 高雄師範大學 === 生物科技系 === 100 === The genome of Burkholderia pseudomallei is highly plasticity because, by geographical isolation, the bacteria endure the different environmental pressure go through rapidly microevolution for a long-term. We hypothesize that, since nitrogen metabolism-related genes shared the conserved characteristics among different species, the genes would be akin in B. pseudomallei isolates. Thus, the whole genome sequences of B. pseudomallei 1710b, 1106a, K96243 and 668 that have published on GenBanks, as well as vgh19, an indigenous strain that have sequenced by us were performed the annotation in following as the manipulation, including (1) determination of the minimal orthologous domain, (2) utilizing traditional initiation codon for translation, (3) promoter appeared in 5’-untranslating region and (4) collinear alignment of prokaryotic genes. TRS (tandem repeated sequence) was recognized as hot spot of mutation rate while 6.81-14.14% of discrimination in B. pseudomallei genome was found. The anticodon nucleotide sequences of tRNAVal, tRNAMet and tRNALys genes are corresponded to codon usage in prokaryotes. The numbers of CDS and ORF appeared in these genes were differences, approximately 3.35% and 44.12% in originals and 1.06% and 20% in the status after annotation in this study. NorB gene was not found in B. pseudomallei vgh19 genome. However, the nucleotide sequences of nitrogen metabolism-related genes were immodestly appeared overlapping, alternative initiation codons and lacks of promoter regions. Totally, 80-83 genes based on orthologous domain were defined. The genes in parts appeared at least 2-5 copies in genomes. The functions of these genes seemed to play a role in ammonification and denitrification, a function in soil bacteria.
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