| Summary: | 碩士 === 國立宜蘭大學 === 園藝學系碩士班 === 100 === This study was to investigate the conditions and methods for Chinese Cymbidium plant regeneration via shoot tip and lateral bud explants in vitro. Four approaches including sterilization of explants, forms of cultural media, cultural conditions and the concentrations of plant growth regulators were adopter. In the sterilization of explants experiment, made use of 1% NaOCl to sterilize the shoot tip for three minutes better than 1.2% NaOCl to sterilize the lateral bus for fifteen minutes have obtained the highest survival rate. In the effects of the concentrations of plant growth regulators experiment, the result showed whether explants were cultivated in 1 mg / l NAA adding with different kinds of plant growth regulators, namely, BA and Kinetin at the concentrations ranged from 0.1 to 1 mg / l, and where was no effect found on the growth of bud. In the forms of cultural medium experiment, the liquid medium culture with a survival rate 67% was higher than the solid medium and had the best performance of the explants in cultivation. Cultivation under light or dark treatment showed the dark treatment with survival rate 83% was higher than light treatment ; under light treatment the rate of browning was 75%. Browning of explants was occurred easier under light treatment more than dark treatment. Illumination enhanced browning velocity of the explants.After cultured in solid medium for three month , the shoot tip explants manifested green color and had bud differentiation and formed a rhizome shape rough surface. This well growth explants were transferred to 0.1 mg / l NAA medium under dark treatment for liquid culture. The result suggest that the explants had white bud and rhizome formation. Expectations of the future they can grow into a complete plant.
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