| Summary: | 碩士 === 國立宜蘭大學 === 動物科技學系碩士班 === 100 === Soybean meal is the most important protein resources in livestock and poultry feed
industry. However, a variety of anti-nutrientional factors, including trypsin inhibitor, globulins
and lectins, etc., the globulins which the main heat stable storage protein of glycinin and
β-conglycinin, which antigenicity to cause hypersensitivity response and damage intestine
morphology by reducing villous height and increasing crypt depth, interfere with digestion,
absorption and utilization of nutrients, especially in young animals. This study was to
investigate fermentation of soybean meal parameter optimized by Aspergillus oryzae and
Bacillus subtilis inoculation, moreover, as alternative protein resources to evaluate effect on
growth performance and anti-soybean allergens-specific antibody titer expression in weaning
piglets. Soybean meal was treated with 3 × 3 factorial experiments which contain 40%, 45%
and 50% moisture, as well as 5% and 10% brown sugar as exogenous carbon resources by A.
oryzae fermentation at 7d. Results showed 10% moisture decrease and lower pH values,
higher hydrolyzed effect in 40% moisture without brown sugar treatment which indicated the
highest protease activity and the least amylase activity. When moisture content of 40% along
with carbon source of 2.5% and 5% brown sugar, glucose, sucrose and starch, respectively, A.
oryzae fermentation showed better mycelium growth performance with 2.5% sucrose and
more higher amylase activity in starch treatment. But better protein hydrolysis was observed
without exogeneous catbon resources incorporated. Similarly, regulation moisture of soybean
meal fermentation by B. subtilis at 100% moisture exhibited higher protease activity and
digestion than 50% moisture. When 2.5% exogenous carbon resources was added at 100%
moisture condition, glucose showed slighyly better amylase activity. Nevertheless, soybean
meal fermentation by B. subtilis showed better hydrolysis without combined with any carbon
resources. The previous experiments revealed A. oryzae fermentation remain contain
abundant of soybean antigens which mainly hydrolyze soybean proteins at 35 kDa compared
with B. subtilis fermentation. Moreover, large scale fermented soybean meal was produced by
B. subtilis fermentative for animal trial. A piglet feeding experiment was performance, and
results showed a tendency towards increase feed intake and weight gain weekly when
compared to control, and piglet feed with fermented soybean meal revealed lower levels of
serum anti-soybean IgG than control at the 2nd and 3rd weeks after feeding. Soybean meal
fermentation bu B. subtilis improve the palatability and results in better growth performance
in weaning piglets, and demonstrate the high potential for fish meal substitution.
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