| Summary: | 碩士 === 國立宜蘭大學 === 食品科學系碩士班 === 100 === In this study sesame seed was soaked with different salt stress for 9 hours, fllowed by 1—6 day germination in dark, germination percentage, seedling
length and composition of seedling were observed, and the variation of phenolics and antioxidant activity were also analysis in the period of
germination.
The results showed that the germination percentage of sesame under 0.9M
NaCl pretreatment was 75.9%, that was the highest in all systems. The systems
of RO and 0.3M NaCl had higher seedling length of 3.47 and 3.63 cm
respectively.
The analysis of proximate composition showed that there was no significant
difference on the content of ash and crude protein in all systems. The ash
content in all systems were between 4.43—5.79%, the protein contents were
between 18.39—22.57%, althought the content of crude protein showed a little
bit higher unter 0.3M and 0.9M salt treatmeats. When compare the change of
crude lipid and carbohydrate of sesame seedling in the period of germination,
the content of the crude lipids 37.04—56.37% decreased while carbohydrates
16.39—37.63% were increase proportionally. Higher carbohydrate content
and lower lipid content were observed in the systems with salt stress than that of RO.
The change of total phenols in sesame seedlings showed that total phenolic
content of sesame seedling under 0.9M NaCl pretreatment were highest, 1.5M
NaCl treatments is next, but there was no significant increased in the system of
0.3M NaCl. Total phenolic content of sesame oil under 0.9M salt pretreatment at
6th days were highest, but the others didn’t have significantly.
When evaluate the change of antioxidation capacity of sesame during
germination, the chelating power was 76.86% first day and the reducing power
was 117.58 mg ascorbic acid / g MeOH extract 5th day had the best
antioxidative activity. The free radical scavenging activity and ORAC of
sesame seedlings were 87.48% and 4280.13μmole TE / g MeOH extract
sepectively for 1.5M system had the highest activity.
For the change of antioxidation capacity of sesame oil in the period of
germination, 0.9M NaCl system had the highest free radical scavenging activity
63.90% and ORAC 524.00μmole TE / g MeOH extract respectively at 2th and
6th day. 1.5M salt treatment of germination sesame oil at 4th day had the best
reducing power, 61.48 mg ascorbic acid / g MeOH extract, and 0.3M treatment
at 4th day of sesame oil had the best chelating power 32.90%.
Keyword: sesame, salt, stress, phenolics
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