The Effect of Quercetin and 1,2,3,4,6-penta-O-galloyl-β-D-glucose (5GG) in Breast Cancer Cells.

• Main Authors: Lee, Szu-Yi, 李思儀
• Other Authors: Hsiu-Chen Huang
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/38gwph

碩士 === 國立新竹教育大學 === 應用科學系碩士班 === 100 === 　　Breast cancer is the most universal cancer in women, but the medications of breast cancer usually cause serious side effects and is no effective treatment for triple-negative breast cancer. Therefore, we wanted to use complementary and alternative medicine (CAM) for reducing the side effects and improving breast cancer treatment. Previous studies showed that the flavonoids quercetin (Que) was a natural product and could inhibit cancer cell growth and the anti-cancer mechanisms of Que was different from galloyl moiety structural analogs. For the reason that we wanted to examine the effect of breast cancer cells co-treated with Que plus GA, EGCG, or 5GG respectively. We found that Que combined with 5GG was most effective in inhibiting triple-negative breast cancer, MDA-BM-231, cell growth. 　　We discovered that combined treatment with Que and 5GG induced cell cycle arrest and apoptosis at different time points, and decreased the protein expressions of cullin 1, CDK4, and SKP2. After SKP2 siRNA transfection for 48 hours, MDA-MB-231 cells showed more apoptotic cells. It has been known that Skp2 plays an important role in regulating G1–S-phase progression. In the present study, we conjectured that RNAi knockdown of SKP2 not only induced cell cycle arrest but also induced apoptosis in MDA-MB-231 cells. Additionally, phosphorylated AKT expression was present at low levels in MDA-MB-231 co-treated with Que and 5GG. We suggested that co-treated with Que and 5GG inhibited MDA-MB-231 cells growth through suppression of the PI3K/Akt pathway. Thus, the combination of Que and 5GG warrants further study as a potential treatment for breast cancer.