A microextraction system for human serum albumin using imprinted polymers

• Main Authors: Wei Li, 李威
• Other Authors: Dar-Fu Tai
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/99112326320642247791

碩士 === 國立東華大學 === 化學系 === 100 === Molecularly imprinted polymers （MIPs） not only can identify small molecules or short peptides, but also can recognize macromolecule, such as protein. However, its performance is still not perfect. Iron oxide nanoparticle is granule possessing large surface area with a suitable binding characteristic. In comparison with other matrix, it can perform separation easily using its magnetic property. Two peptides in the flank part of the human serum albumin （HSA） spatial structure were selected as the template. One is a random coil peptide and the other has a helical structure. These templates were synthesized using microwave-assisted method. As iron oxide nano-particle was subjected to several modification steps, MIPs were then fabricated on the magnetic particle by heating with the template. Kinetic analysis confirmed the affinity of these MIPs toward HSA,　adsorption were able to operate at very low concentration. The Kd values derived from random coil and helix were measured as 12.12 nM and 2.10 nM, respectively. These MIPs magnetic particles were also proved to be feasible for at least four operations.