The Study of Detachment Mechanism of Single Cell on Substrate Using the Dielectrophoresis Force and Trypsin Enzyme

• Main Authors: Wei-Ping Chen, 陳尉平
• Other Authors: Chyung Ay
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/25346414747273969571

碩士 === 國立嘉義大學 === 生物機電工程學系研究所 === 100 === The study of cell biology has developed several methods of capturing specific single cell; then, cell proliferation were used to obtain large quantities of the cell. This study takes cell (human chondrocytes, SW1353) to be cultured in the biomaterial PDMS substrate; a microscope with CCD and PCI image card are used to show the images of cell morphology to be observed on the video camera. Specific single cell is selected first, then three kinds of different concentration (0.1%, 0.15%, 0.2%) of trypsin are added, and three kinds of shape in electrode design (convex, wavy, serrate) of dielectrophoresis (DEP) force is applied to induce the cells detach from the substrate. This study will try to find the optimal conditions and methods of trypsin concentration or DEP force to detach the cell. In this study, we find that the better condition of trypsin concentration is 0.2% and the detachment rate of cell is 29.03%. The fastest cell detachment time is the dielectrophoresis force due to the wavy shape electrode. But the optimal conditions and methods is the serrate shape electrode of dielectrophoresis force which the cell detachment rate is the highest about 83.33%. However, it also owns the highest survival rate without lysis about 60%.