| Summary: | 碩士 === 國立嘉義大學 === 生物農業科技學系碩士班 === 100 === Abstract
Rice is a staple source of food for human consumption. Drought stress is one of the most important constraints limiting rice production in most rain-fed systems worldwide. The molecular mechanism for resisting drought condition for plants is a complicated process in which the expression of numerous genes can be induced and regulated. For investigating the pattern of genome-level gene expression, some strategies such as microarray, RNAi, or epigenetic detection can be used. Recently epigenetic studies on genomic DNA methylation for gene regulation have been accepted as a popular and powerful tool. In this study, we aim to detect DNA methylation differences in four rice genotypes with different responses of drought tolerance using methylation-sensitive amplification polymorphism (MSAP). The result indicates that drought induced DNA methylation alteration are specific to genotype and organ types. The high frequency of alteration in methylated and demethylated sequences suggests that many genes may be affected. Further analysis suggested that demethylation of some genes occurs under drought stress, which triggers a protective mechanism by turning on their gene expression. In our epigenetic study, our results can provide the molecular understanding of the adaptation mechanism to drought stress in rice.
|
|---|
