Axillary shoot multiplication by using stalk node explants for new Phalaenopsis hybrids

• Main Authors: Thi-Nga Hoang, 黃氏雅
• Other Authors: 徐善德 博士
• Format: Others
• Language: en_US
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/49749048782434830999

碩士 === 國立嘉義大學 === 園藝學系研究所 === 100 === This study aimed to evaluate the effects of basal medium, light quality, and cytokinins on shoot formation from stalk node explants of new Phalaenopsis hybrids bred by National Chiayi University, Chiayi, Taiwan. The effect of basal medium on shoot formation from stalk node explants of P. Chiada Spark ‘102#10’ by using Murashige and Skoog (MS) culture medium of different strength (1/4, 1/3, 1/2 and full strength) supplemented with 3 mg.L-1 6-benzylaminopurine (BA) was investigated firstly. The results showed that 1/2 MS was the best basal medium for shoot formation and it was therefore chosen for further use in this study. The effect of light quality on shoot formation from stalk node explants of P. Chiada Spark ‘102#10’ was investigated by using the Mini-E-Light system (Nano Bio Light Technology Co., Ltd., New Taipei, Taiwan) equipped with light-emitting diodes (LEDs) as light source and included with 8 types of light conditions: warm white light (WW), cold white light (CW), 9 blue light (9B), 9 red light (9R), 8 red + 1 blue light (8R1B), 7 red + 1 blue + 1 far-red light (7R1B1IR), 7 red + 1 green + 1 blue light (7R1G1B), and 3 red + 3 blue + 3 far-red light (3R3B3IR) (see details in content). After 200 days of culture, 7R1B1IR, 7R1G1B, and 9R showed the most effectiveness on the number of shoots per explant and fresh weight of culture, while 9B exhibited the lowest on both of them. The effect of cytokinins on shoot formation from stalk node explants by using 1/2 MS culture medium with thidiazuron (TDZ) added at the concentrations of 0, 0.01, 0.1, and 1.0 mg•L-1 in combination with BA at 0, 0.1, 1.0, and 10 mg•L-1 was investigated in Doritaenopsis Chiada Gloria. The highest shoot formation percentage (79.2%) with two or more shoots per explant was obtained by TDZ at 0.1 mg.L-1 with BA at 1.0 mg.L-1. The highest protocorm-like body (PLB) like structure formation (37.5 %) was obtained when medium was supplemented with 1.0 mg.L-1 TDZ alone. For shoot formation from stalk node explants of new Phalaenopsis hybrids experimented in this study, data suggest that 1/2 MS medium supplemented with 3 mg.L-1 BA under 9R single light or 7R1B1IR mixture light condition, or 1/2 MS medium supplemented with 0.1 mg.L-1 TDZ and 1.0 mg.L-1 BA under florescent lamp are the best combinations.