The Primary Growth and Triterpene Analysis of Antrodia cinnamomea Cultured on Branch-Based Substrates of Cinnamomum camphora (L.) Presl. and Acacia confusa Merr.

• Main Authors: YuLing Hsieh, 謝育玲
• Other Authors: 廖宇賡
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/13018163258046747228

碩士 === 國立嘉義大學 === 森林暨自然資源學系研究所 === 100 === This study deals with culturing Antrodia cinnamomea TT Chang ＆WN Chou in vitro by using wood material of Cinnamomum camphora (L.) Presl. (Cc) and Acacia confuse Merr. (Ac), collected from debarked branches of living trees as incubation substrate. The same collection of C. kanehirae Hay. (Ck) was also included as control. These three wood materials were processed into small logs (with diameter less than 7.0 cm), blocks (2 × 2 × 2 cm3) and fine chips to be further shaped into cubic cakes (6 × 6 × 6 cm3). Preliminary observations showed that the hypha of A. cinnamomea grew well on Cc and Ac substrates (block medium and logs) and simultaneously covered the substrate surface within 4 or 8 weeks, respectively. As compared with fully (100%) hyphal coverage on Ck cake (control), hyphal growth on Cc and Ac cakes was slower, with 80% surface coverage. The primordia of A. cinnamomea was observed on shaped cakes for 4-month culture. The clamp cells were also observed on the A. cinnamomea hypha (the inoculant) incubated on medium plate for 8 weeks. This evidence revealed that the inoculant cultures were developing into generative hypha, and capable of forming fruiting body. The HPLC analysis of wood powder and A. cinnamomea extraction demonstrated that the wood material of all species contain no triterpenes specifically determined in A. cinnamomea. The A. cinnamomea and its primordia revealed that the bio-synthesis of lanostane-type triterpenes, like dehydroeburicoic acid, 15α-acetyl dehydrosulphurenic acid, 3β, 15α-dihydroxy lanosta-21-oic acid, dehydrosulphurenic acid and sulphurenic acid, was independent of culture substrates. Although the primordia was obtained from 9-month-old cultures, its growth stage was identified as in a transition status prior to the fruiting body formation due to the absence of fruiting-body ergostane-type triterpenes. The culture system presented in this study does not fully fill the requirement of forming fruiting body during the 9-month culture period.