Effects of Different Sawdust Media on Growth and Constituents of Antrodia cinnamomea

• Main Authors: Bo-Ya Tsai, 蔡博雅
• Other Authors: Ming-Jen Lee
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/04063427253047585680

碩士 === 國立嘉義大學 === 森林暨自然資源學系研究所 === 100 === Abstract Antrodia cinnamomea Chang and Chou, a heart-rot decaying fungus of stout camphor (Cinnamomum kanehirae Hayata), belonging to genus Antrodia, family Polyporaceae, is an endemic fungal species in Taiwan. Stout camphor has become less and less due to illegal cutting for harvesting and culturing fruiting body in past decades. Thus, it’s getting difficult to harvest fruiting bodies of A. cinnamomea from natural forest. 　　In this study, strains of A. cinnamomea were isolated, identified and cultured in different sawdust media. The effects of different media on growth and constituents of strains of A. cinnamomea were investigated in order to provide more information for cultivating A. cinnamomea. 　　The results showed that the isolated strains were identified as A. cinnamomea via hyphal morphology and ribosomal DNA 5.8S sequence analysis. The results revealed that the growth of A. cinnamomea in Taiwan acacia (Acacia confusa Merr.) sawdust media was higher than that in camphor tree (Cinnamomum camphora (L.) Sieb) sawdust media. Meanwhile, addition of 10% rice bran and leave powder of stout camphor tree into the sawdust media promoted mycelia growth of strains of A. cinnamomea and and Improved the content of , sulphurenic acid, eburicoic acid and polysaccharide 　　Genetic study of A. cinnamomea from Liouguei and Hualien by AFLP (amplified fragment length polymorphism) and UPGMA (unweighted pair-group method with arithmetic mean) techniques showed that A. cinnamomea of different regions could be divided into two clusters. A. cinnamomea from different regions showed different genetic diversity.