Tissue culture propagation of Drosera burmannii Vahl: a conserved plant species in Kinmen

• Main Authors: Ya Yin Ji, 季亞垠
• Other Authors: Yue Ken Liao
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/44900384563134636293

碩士 === 國立嘉義大學 === 森林暨自然資源學系研究所 === 100 === The present study has developed a tissue culture protocol incorporating the induction of shoot fasciation in Drosera burmannii Vahl for mass propagation of this plant species conserved in Kinmen. The explants (shoot tips) exhibited 50% of fasciated plant (FP) formation after being induced by 20 µM 6-furfurylaminopurine (kinetin). The anatomical analysis revealed that there are multiple apical meristems arranged perpendicularly to the longitude section of FP apical stem. When a 0.5 cm wide piece of the FP was excised and cultured on the cytokinin-free 1/2 MS medium, it proliferated with its width continuously extended every 30 days (one subculture). However, the greatest width increase (4.38 ± 0.11 cm per subculture) was obtained from 20 µM 6-benzylaminopurine (BAP) induced FP. Such events of FP to proliferate and maintain fasciated characteristics during subcultures prolonged for 270 days (nine subcultures) without apparent changes. The termination of FP subculture was possible when dark incubation was applied for 60 days followed by fine mincing of the spindling FP. Morphologically normal plants were recovered from these operations and the maximal production of normal plants (20.1 ± 1.8 plants per 1.0 cm width of spindling FP) was obtained from 20 µM BAP induced FP. An estimated production of normal plants, initiated from a 0.5 cm piece of FP, was thus achieved to the amount of 3.2 × 1010 within one year (270-day FP subculture plus 60-day dark spindling and 45-day normal plant recovery cultures).