Study on the optimal operation condition of Panax ginseng fractioned by supercritical carbon dioxide and antioxidant activity of fractions

• Main Authors: I-Lung Yu, 游宜龍
• Other Authors: Shu-Mei Lin
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/28304552465327457087

碩士 === 國立嘉義大學 === 食品科學系研究所 === 100 === Panax ginseng C.A. Meyer, with thousands of years of history, has been known as a valuable traditional Chinese medicine for thousands of years. The main components in ginseng, including ginsenosides and polysaccharides, exert the capability to inhibit the proliferation of tumor cells, regulate immune system, and prevent fatigue. Solubility of the substances varies depending on operating conditions, such as pressure and temperature, in the supercritical fluid system. The primary objective of this study is to investigate the optimal operation conditions for separation of polysaccharides and ginsenosides from ginseng extract in fraction (F) and residual (R) fractions using supercritical carbon dioxide fractionation technique. Panax ginseng was mixed with 80% ethanol at a ratio of 1:4 (v/w) for 24 h to yield ethanol extract. An orthogonal design was performed under the following operating parameters, including temperature (40, 50 and 60 oC), pressure (10, 20 and 30 MPa), the supercritical CO2 flow rate (2, 4 and 6 ml/min), and flow rate of ginseng extract liquor (1, 2 and 3 ml/min). The compositions, including ginsenosides, polysaccharides, malondialdehyde (MDA), and the free radical scavenging ability of ginseng extract and fractions were determined. The analytical data show that the highest selectivity of ginsenosides and polysaccharides and the lowest ration of MDA content were operated at high temperature and pressure. The polysaccharides and ginsenosides content in fraction were 0.149 g/100g dw and 0.563 g/100g dw when fractioned at 30MPa, 40oC, 6 ml/min CO2 flow rate and 3 ml/min ginseng exctract flow rate, respectively. The SC50 of DPPH and ABTS+ of fraction were 3.35 g/l, and 3.68 g/l, respectively.