| Summary: | 博士 === 國立中央大學 === 機械工程研究所 === 100 === Cell migration has various effects on the biological phenomena, such as wound healing, metastasis, and invasion. It is even involved in embryogenesis. Cell migration is one of the most important processes in physiology and pathology. The Boyden chamber can provide a stable gradient distribution and is commonly used to estimate cell motility. A Boyden chamber assay can be used to easily determine the chemotaxis of various chemicals.
In this study, we examined the response of placenta-derived multipotent cells (PDMCs) on the collagen type I. We used a Boyden chamber to estimate the chemotactic responses of the cells to Type I collagen, and determined the integrins on the cell membrane using a flow cytometer. We concurrently developed a mathematical model to investige the process of the chemotaxis experiment that includes the chemoattractant transpont in the Boyden chamber assay. In addition, we used the Lapidus-Schiller equation to model cells’ random walk and chemotactic motility that combined with the cell sedimentation in the upper well of the Boyden chamber. Furthermore, we used a more detailed mathematical model to describe the chemotactic response. The response between the receptor and the chemoattratant was used to simulate chemotactic migration. Results show that once cell sedimentation is involved, the assumption of a quasi-steady receptor distribution by the Lapidus-Schiller equation may be invalid for the Boyden chamber assay. This is because the formation of the chemical-receptor complexes is profoundly delayed by the process of cell sedimentation in the upper well of the Boyden chamber.
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